Systematic Analysis of Two Tandem GGDEF/EAL Domain Genes Regulating Antifungal Activities in Pseudomonas glycinae MS82

Cyclic diguanylate (c-di-GMP) affects bacterial physiological and biochemical functions like biofilm, motility, virulence, secretion systems. GGDEF/EAL-domain proteins, participating in c-di-GMP synthesis degradation, are widely present Pseudomonas, with various structures functions. Pseudomonas glycinae MS82 is a rhizosphere bacterium that protects mushroom against the pathogenic fungi. Although 14 genes encoding proteins have been identified genome of MS82, regulation poorly understood as facilitator or repressor phenotypes. Here, PafQ PafR, tandem GGDEF/EAL domain, were functionally analyzed found to regulate antifungal activity. Individual deletion mutants PafR constructed P. through biparental conjugation homologous recombination. Subsequently, activity, biofilm formation, expression related substance examined contrasted those wild-type MS82. Most phenotypes activities significantly reduced after knocking out PafR. In other members genus possess different regulation. glycinae, positive on fungistatic synthesis, motility crucial biocontrol diseases.